Validation of the PCR-dHPLC methodology for fast identification of Candida glabrata phylogenetically associated species in several organic matrices. Since two new species phylogenetically associated to Candida glabrata with barely completely different phenotypes and antifungal susceptibility profiles have been described, it appears to be crucial from medical viewpoint, to develop a fast and correct identification system with […]
Development of a new DHPLC assay
Growth of a brand new DHPLC assay for genotyping UGT1A (TA)n polymorphism related to Gilbert’s syndrome. BACKGROUND Gilbert’s syndrome is the commonest hereditary dysfunction of bilirubin metabolism. The causative mutation in Caucasians is sort of solely a (TA) dinucleotide insertion within the UGT1A1 promoter. Affected people are homozygous for the variant promoter and have 7 TA repeats […]
Application of DHPLC screening TGFBR-3 gene
[Application of DHPLC screening TGFBR-3 gene in Chinese women with idiopathic premature ovarian failure]. OBJECTIVE To judge scientific worth of denaturing excessive efficiency liquid chromatography (DHPLC) utilized in detecting remodeling development issue beta receptor 3 (TGFBR-3) exons 11 and 12 polymorphism in ladies with idiopathic untimely ovarian failure (POF). METHODS From Feb. 2009 to Dec. 2011, 110 […]
Detection of mutations in gyrB using denaturing
Detection of mutations in gyrB using denaturing extreme effectivity liquid chromatography (DHPLC) amongst Salmonella enterica serovar Typhi and Paratyphi A. Fluoroquinolone resistance is mediated by mutations inside the quinolone-resistance determining space (QRDR) of the topoisomerase genes. Denaturing extreme effectivity liquid chromatography (DHPLC) was evaluated for detection of clinically very important mutations in gyrB amongst Salmonella. […]
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