Validation of the PCR-dHPLC methodology for fast identification of Candida glabrata phylogenetically associated species in several organic matrices. Since two new species phylogenetically associated to Candida glabrata with barely completely different phenotypes and antifungal susceptibility profiles have been described, it appears to be crucial from medical viewpoint, to develop a fast and correct identification system with […]
COLD-PCR, DHPLC and GeneScanning
The use of COLD-PCR, DHPLC and GeneScanning for the highly sensitive detection of c-KIT somatic mutations in canine mast cell tumours. The conventional polymerase chain reaction (PCR)/sequencing methods may be poorly suited for the detection of somatic mutations in canine mast cell tumour (MCT) samples owing to limited sensitivity. This study was aimed at establishing novel […]
Detection of mutations in gyrB using denaturing
Detection of mutations in gyrB using denaturing extreme effectivity liquid chromatography (DHPLC) amongst Salmonella enterica serovar Typhi and Paratyphi A. Fluoroquinolone resistance is mediated by mutations inside the quinolone-resistance determining space (QRDR) of the topoisomerase genes. Denaturing extreme effectivity liquid chromatography (DHPLC) was evaluated for detection of clinically very important mutations in gyrB amongst Salmonella. […]
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