COLD-PCR, DHPLC and GeneScanning

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long-qt-syndrome

The use of COLD-PCR, DHPLC and GeneScanning for the highly sensitive detection of c-KIT somatic mutations in canine mast cell tumours.

 

The conventional polymerase chain reaction (PCR)/sequencing methods may be poorly suited for the detection of somatic mutations in canine mast cell tumour (MCT) samples owing to limited sensitivity. This study was aimed at establishing novel and more sensitive methods, assessing their limit of detection and comparing their sensitivity with conventional methods.Two different ‘driver’ somatic mutations of c-KIT, together with the wild-type counterparts, were cloned in plasmids to prepare standard samples with known concentrations of mutated alleles in a background of wild-type alleles; the plasmids standards were assayed using either conventional or novel, highly sensitive technique.

Conventional PCR/sequencing showed a sensitivity of 50-20%. Conversely, all the novel methods obtained higher sensitivities allowed reaching as low as 2.5-1.2% of the mutated DNA.The study demonstrates that early conventional methods could likely have underestimated the prevalence of KIT mutations of MCTs, therefore affecting the assessment of their relevance in prognosis and tyrosine kinase inhibitor (TKI) treatment effectiveness.

Efficient IDUA Gene Mutation Detection with Combined Use of dHPLC and Dried Blood Samples.

Objectives. Development of a simple mutation directed method in order to allow lowering the cost of mutation testing using an easily obtainable biological material. Assessment of the feasibility of such method was tested using a GC-rich amplicon. Design and Methods. A method of denaturing high-performance liquid chromatography (dHPLC) was improved and implemented as a technique for the detection of variants in exon 9 of the IDUA gene. The optimized method was tested in 500 genomic DNA samples obtained from dried blood spots (DBS).

Results. With this dHPLC approach it was possible to detect different variants, including the common p.Trp402Ter mutation in the IDUA gene. The high GC content did not interfere with the resolution and reliability of this technique, and discrimination of G-C transversions was also achieved. Conclusion. This PCR-based dHPLC method is proved to be a rapid, a sensitive, and an excellent option for screening numerous samples obtained from DBS. Furthermore, it resulted in the consistent detection of clearly distinguishable profiles of the common p.Trp402Ter IDUA mutation with an advantageous balance of cost and technical requirements.

 

long-qt-syndrome
long-qt-syndrome

Mouse Filaggrin (FLG) ELISA Kit

DLR-FLG-Mu-48T 48T
EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Filaggrin (FLG) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit

DLR-FLG-Mu-96T 96T
EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Filaggrin (FLG) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit

RDR-FLG-Mu-48Tests 48 Tests
EUR 557

Mouse Filaggrin (FLG) ELISA Kit

RDR-FLG-Mu-96Tests 96 Tests
EUR 774

Recombinant Filaggrin (FLG)

4-RPJ103Hu01
  • EUR 816.80
  • EUR 322.00
  • EUR 2788.00
  • EUR 996.00
  • EUR 1892.00
  • EUR 610.00
  • EUR 6820.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Filaggrin expressed in: E.coli

Recombinant Filaggrin (FLG)

4-RPJ103Mu01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Filaggrin expressed in: E.coli

Human Filaggrin (FLG)

1-CSB-YP008712HU
  • EUR 430.00
  • EUR 234.00
  • EUR 1508.00
  • EUR 642.00
  • EUR 1009.00
  • EUR 291.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Filaggrin(FLG),partial expressed in Yeast

Human Filaggrin (FLG)

1-CSB-BP008712HU
  • EUR 840.00
  • EUR 332.00
  • EUR 2170.00
  • EUR 1135.00
  • EUR 1579.00
  • EUR 470.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Filaggrin(FLG) ,partial expressed in Baculovirus

Human Filaggrin (FLG)

1-CSB-EP008712HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Filaggrin(FLG),partial expressed in E.coli

Filaggrin (FLG) Antibody

20-abx109789
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Filaggrin (FLG) Antibody

20-abx339302
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Filaggrin (FLG) Antibody

20-abx339766
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Filaggrin (FLG) Antibody

20-abx100249
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Filaggrin (FLG) Antibody

20-abx100250
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Filaggrin (FLG) Antibody

20-abx133148
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Filaggrin (FLG) Antibody

20-abx176473
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug

Filaggrin (FLG) Antibody

20-abx172440
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug

Human Filaggrin (FLG) Protein

20-abx066657
  • EUR 1121.00
  • EUR 411.00
  • EUR 3724.00
  • EUR 1358.00
  • EUR 759.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Anti-Filaggrin/FLG Antibody

PB9870 100ug/vial
EUR 334

Filaggrin (FLG) Antibody (FITC)

20-abx106841
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Filaggrin (FLG) Antibody (HRP)

20-abx108261
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Filaggrin (FLG) Antibody (Biotin)

20-abx105422
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Mouse Filaggrin (FLG) Protein

20-abx066658
  • EUR 732.00
  • EUR 286.00
  • EUR 2305.00
  • EUR 885.00
  • EUR 523.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Filaggrin (FLG) Antibody (FITC)

20-abx271155
  • EUR 495.00
  • EUR 258.00
  • EUR 1455.00
  • EUR 676.00
  • EUR 398.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Filaggrin (FLG) Antibody (Biotin)

20-abx271421
  • EUR 467.00
  • EUR 244.00
  • EUR 1344.00
  • EUR 634.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Filaggrin (FLG) Antibody (Biotin)

20-abx272419
  • EUR 453.00
  • EUR 244.00
  • EUR 1316.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Filaggrin (FLG) Antibody (FITC)

20-abx273568
  • EUR 481.00
  • EUR 244.00
  • EUR 1414.00
  • EUR 662.00
  • EUR 356.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Filaggrin (FLG)CLIA Kit

SCJ103Hu-10x96wellstestplate 10x96-wells test plate
EUR 5647.8
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids.

Human Filaggrin (FLG)CLIA Kit

SCJ103Hu-1x48wellstestplate 1x48-wells test plate
EUR 552.76
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids.

Human Filaggrin (FLG)CLIA Kit

SCJ103Hu-1x96wellstestplate 1x96-wells test plate
EUR 746.8
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids.

Human Filaggrin (FLG)CLIA Kit

SCJ103Hu-5x96wellstestplate 5x96-wells test plate
EUR 3060.6
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids.

Human Filaggrin (FLG) CLIA Kit

4-SCJ103Hu
  • EUR 5698.00
  • EUR 3061.00
  • EUR 747.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Filaggrin (FLG)Tissue homogenates and other biological fluids

Filaggrin (FLG) Polyclonal Antibody (Human)

4-PAJ103Hu01
  • EUR 247.00
  • EUR 2510.00
  • EUR 625.00
  • EUR 310.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG)

Human Filaggrin (FLG) ELISA Kit

SEJ103Hu-10x96wellstestplate 10x96-wells test plate
EUR 4731.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Filaggrin (FLG) ELISA Kit

SEJ103Hu-1x48wellstestplate 1x48-wells test plate
EUR 477.3
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Filaggrin (FLG) ELISA Kit

SEJ103Hu-1x96wellstestplate 1x96-wells test plate
EUR 639
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Filaggrin (FLG) ELISA Kit

SEJ103Hu-5x96wellstestplate 5x96-wells test plate
EUR 2575.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Filaggrin (FLG) ELISA Kit

4-SEJ103Hu
  • EUR 4782.00
  • EUR 2526.00
  • EUR 640.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Filaggrin (FLG) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Filaggrin(FLG) ELISA kit

CSB-EL008712HU-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human Filaggrin (FLG) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Filaggrin(FLG) ELISA kit

1-CSB-EL008712HU
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Filaggrin(FLG) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human FLG/ Filaggrin ELISA Kit

E0920Hu 1 Kit
EUR 571

Human Filaggrin (FLG)ELISA Kit

201-12-2700 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Filaggrin (FLG) CLIA Kit

20-abx190272
  • EUR 7911.00
  • EUR 4215.00
  • EUR 973.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Filaggrin (FLG) ELISA Kit

abx572526-96tests 96 tests
EUR 668

Human Filaggrin (FLG) ELISA Kit

20-abx151555
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Filaggrin ELISA Kit (FLG)

RK01403 96 Tests
EUR 521

Human Filaggrin(FLG)ELISA Kit

QY-E01412 96T
EUR 374

Human FLG(Filaggrin) ELISA Kit

EH1353 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human Filaggrin, FLG ELISA KIT

ELI-03920h 96 Tests
EUR 824

Filaggrin (FLG) Polyclonal Antibody (Human), APC

4-PAJ103Hu01-APC
  • EUR 345.00
  • EUR 3275.00
  • EUR 912.00
  • EUR 440.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with APC.

Filaggrin (FLG) Polyclonal Antibody (Human), Biotinylated

4-PAJ103Hu01-Biotin
  • EUR 311.00
  • EUR 2460.00
  • EUR 727.00
  • EUR 381.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with Biotin.

Filaggrin (FLG) Polyclonal Antibody (Human), Cy3

4-PAJ103Hu01-Cy3
  • EUR 419.00
  • EUR 4325.00
  • EUR 1175.00
  • EUR 545.00
  • EUR 251.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with Cy3.

Filaggrin (FLG) Polyclonal Antibody (Human), FITC

4-PAJ103Hu01-FITC
  • EUR 296.00
  • EUR 2640.00
  • EUR 750.00
  • EUR 372.00
  • EUR 195.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with FITC.

Filaggrin (FLG) Polyclonal Antibody (Human), HRP

4-PAJ103Hu01-HRP
  • EUR 316.00
  • EUR 2855.00
  • EUR 807.00
  • EUR 398.00
  • EUR 206.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with HRP.

Filaggrin (FLG) Polyclonal Antibody (Human), PE

4-PAJ103Hu01-PE
  • EUR 296.00
  • EUR 2640.00
  • EUR 750.00
  • EUR 372.00
  • EUR 195.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with PE.

ELISA kit for Human FLG (Filaggrin)

ELK3513 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Filaggrin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit

SEJ103Mu-10x96wellstestplate 10x96-wells test plate
EUR 4862.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit

SEJ103Mu-1x48wellstestplate 1x48-wells test plate
EUR 488.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit

SEJ103Mu-1x96wellstestplate 1x96-wells test plate
EUR 654.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit

SEJ103Mu-5x96wellstestplate 5x96-wells test plate
EUR 2644.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit

4-SEJ103Mu
  • EUR 4913.00
  • EUR 2595.00
  • EUR 655.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Filaggrin (FLG) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Filaggrin(FLG) ELISA kit

CSB-EL008712MO-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Mouse Filaggrin (FLG) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Filaggrin(FLG) ELISA kit

1-CSB-EL008712MO
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse Filaggrin(FLG) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Filaggrin ELISA Kit (FLG)

RK02813 96 Tests
EUR 521

Mouse Flg/ Filaggrin ELISA Kit

E0543Mo 1 Kit
EUR 571

Mouse Filaggrin (FLG) ELISA Kit

abx573034-96tests 96 tests
EUR 668

Mouse Filaggrin (FLG) CLIA Kit

20-abx495617
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Filaggrin (FLG) ELISA Kit

20-abx154027
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Filaggrin (FLG) ELISA Kit

abx257768-96tests 96 tests
EUR 746

Mouse Filaggrin(FLG)ELISA Kit

QY-E21727 96T
EUR 374

Mouse FLG(Filaggrin) ELISA Kit

EM1853 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 9.375pg/ml

Mouse Filaggrin, Flg ELISA KIT

ELI-03919m 96 Tests
EUR 865

Filaggrin (FLG) Polyclonal Antibody (Human), APC-Cy7

4-PAJ103Hu01-APC-Cy7
  • EUR 571.00
  • EUR 6430.00
  • EUR 1705.00
  • EUR 760.00
  • EUR 319.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with APC-Cy7.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat)

4-PAJ103Mu01
  • EUR 251.00
  • EUR 2576.00
  • EUR 640.00
  • EUR 316.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG)

ELISA kit for Mouse FLG (Filaggrin)

ELK6992 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Filaggrin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human FLG-Ab (Anti-Filaggrin)

E-EL-H0283 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human FLG-Ab (Anti-Filaggrin) in samples from Serum, Plasma, Cell supernatant

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), APC

4-PAJ103Mu01-APC
  • EUR 351.00
  • EUR 3365.00
  • EUR 935.00
  • EUR 449.00
  • EUR 222.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with APC.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), Biotinylated

4-PAJ103Mu01-Biotin
  • EUR 316.00
  • EUR 2526.00
  • EUR 744.00
  • EUR 387.00
  • EUR 221.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with Biotin.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), Cy3

4-PAJ103Mu01-Cy3
  • EUR 427.00
  • EUR 4445.00
  • EUR 1205.00
  • EUR 557.00
  • EUR 254.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with Cy3.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), FITC

4-PAJ103Mu01-FITC
  • EUR 301.00
  • EUR 2712.00
  • EUR 768.00
  • EUR 379.00
  • EUR 197.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with FITC.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), HRP

4-PAJ103Mu01-HRP
  • EUR 321.00
  • EUR 2933.00
  • EUR 827.00
  • EUR 405.00
  • EUR 209.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with HRP.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), PE

4-PAJ103Mu01-PE
  • EUR 301.00
  • EUR 2712.00
  • EUR 768.00
  • EUR 379.00
  • EUR 197.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with PE.

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561) Antibody

BNC041561-100 100uL
EUR 199
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561),CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561) Antibody

BNC041561-500 500uL
EUR 544
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561),CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562) Antibody

BNC041562-100 100uL
EUR 199
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562),CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562) Antibody

BNC041562-500 500uL
EUR 544
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562),CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563) Antibody

BNC041563-100 100uL
EUR 233
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563), CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563) Antibody

BNC041563-500 500uL
EUR 545
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563), CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945) Antibody

BNC041945-100 100uL
EUR 233
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945), CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945) Antibody

BNC041945-500 500uL
EUR 545
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945), CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R) Antibody

BNC041957-100 100uL
EUR 233
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R), CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R) Antibody

BNC041957-500 500uL
EUR 545
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R), CF405S conjugate, Concentration: 0.1mg/mL

Use of PCR-DHPLC with fluorescence detection for the characterization of the bacterial diversity during cassava (Manihot esculenta Crantz) fermentation.

 

  • Denaturing high-performance liquid chromatography (DHPLC) has been described as a suitable method to study DNA polymorphisms. Here, cassava (Manihot esculenta Crantz) fermentation liquor was examined using DHPLC analysis to characterize the bacterial diversity during the fermentation process. GC-clamped amplicons corresponding to a variable region of the bacterial community 16S rDNA were synthesized using polymerase chain reaction (PCR) and then resolved on a base-composition basis using preparative DHPLC.
  • Eluate fractions were collected at random and used as a source of whole community DNA that could be used to determine the bacterial diversity. As a first approach, GC-clamps were removed from the eluted DNA fragments using PCR to avoid the possible bias these clamps could cause during the construction of clone libraries. As a second approach, a clone library of each eluate sample was constructed, preserving the GC-clamps of the DNA fragments.
  • The first approach generated 132 bacterial rDNA sequences with an average size of 200 bp, 45% of which had similarity to unculturable or non-classified bacteria. The second approach produced 194 sequences identified as Proteobacteria (48%), uncultured or non-classified environmental bacteria (40%) and Firmicutes (12%).

We detected a remarkably greater bacterial diversity using the first approach than the second approach. The DHPLC-PCR method allowed for the fast and non-laborious detection of a vast bacterial diversity that was associated with cassava fermentation, and we conclude that it is a promising alternative for the characterization of the overall microbial diversity in complex samples.

 

Use of denaturing high-performance liquid chromatography (DHPLC) to characterize the bacterial and fungal airway microbiota of cystic fibrosis patients.

 

The aim of this study was to evaluate the use of denaturing high-performance liquid chromatography (DHPLC) to characterize cystic fibrosis (CF) airway microbiota including both bacteria and fungi. DHPLC conditions were first optimized using a mixture of V6, V7 and V8 region 16S rRNA gene PCR amplicons from 18 bacterial species commonly found in CF patients.

Then, the microbial diversity of 4 sputum samples from 4 CF patients was analyzed using cultural methods, cloning/sequencing (for bacteria only) and DHPLC peak fraction collection/sequencing. DHPLC analysis allowed identifying more bacterial and fungal species than the classical culture methods, including well-recognized pathogens such as Pseudomonas aeruginosa.

Even if a lower number of bacterial Operational Taxonomic Units (OTUs) was identified by DHPLC, it allowed to find OTUs unidentified by cloning/sequencing. The combination of both techniques permitted to correlate the majority of DHPLC peaks to defined OTUs.

Finally, although Aspergillus fumigatus detection using DHPLC can still be improved, this technique clearly allowed to identify a higher number of fungal species versus classical culture-based methods. To conclude, DHPLC provided meaningful additional data concerning pathogenic bacteria and fungi as well as fastidious microorganisms present within the CF respiratory tract. DHPLC can be considered as a complementary technique to culture-dependent analyses in routine microbiological laboratories.

 

DHPLC and MS studies of a photoinduced intrastrand cross-link in DNA labeled with 5-bromo-2′-deoxyuridine.

 

  • It is well known that the replacement of thymidine with 5-bromo-2′-deoxyuridine (BrdU) in DNA sensitizes it to UVB light. Irradiation of a biopolymer substituted in such a way leads to manifold kinds of DNA damage, such as intrastrand cross-links, single- and double-strand breaks or alkali-labile sites that were studied in the past with a broad spectrum of analytical methods.
  • Here, we demonstrate that completely denaturing high-performance liquid chromatography (DHPLC), underestimated so far in DNA damage studies, could act as an inexpensive, and high-resolution substitute for the commonly employed gel electrophoresis.
  • We report on the DHPLC/mass spectrometry (MS) analyses of photolytes obtained with the UV irradiation of aqueous solutions containing 40 base pairs of a long, double-stranded oligonucleotide labeled with BrdU in one of its strands.
  • The UV-product was detected by HPLC at a temperature of 70°C. Subsequent MS analysis with electrospray ionization (ESI-MS) of the photolyte, enzymatic digestion of the irradiated material and HPLC and MS analysis (LC-MS) of the digest demonstrated unequivocally that an intrastrand covalent dimer, involving adenine and uracil, is formed in the irradiated system.

CD44 Antigen (CD44) Antibody

abx139276-01mg 0.1 mg
EUR 370

CD44 Antigen (CD44) Antibody

abx139277-01mg 0.1 mg
EUR 370

CD44 Antigen (CD44) Antibody

20-abx007560
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

CD44 Antigen (CD44) Antibody

abx011738-100ug 100 ug
EUR 439

CD44 Antigen (CD44) Antibody

abx025270-400ul 400 ul
EUR 523

CD44 Antigen (CD44) Antibody

abx025270-80l 80 µl
EUR 286

CD44 Antigen (CD44) Antibody

abx015801-100ul 100 ul
EUR 411

CD44 Antigen (CD44) Antibody

20-abx019050
  • EUR 342.00
  • EUR 77.00
  • 100 ug
  • 5 ug

CD44 Antigen (CD44) Antibody

abx021434-02mg 0.2 mg
EUR 537

CD44 Antigen (CD44) Antibody

abx021435-02mg 0.2 mg
EUR 537

CD44 Antigen (CD44) Antibody

abx412639-025mg 0.25 mg
EUR 565

CD44 Antigen (CD44) Antibody

abx412640-25ug 25 ug
EUR 272

CD44 Antigen (CD44) Antibody

abx412685-01mg 0.1 mg
EUR 453

CD44 Antigen (CD44) Antibody

abx412739-01mg 0.1 mg
EUR 453

CD44 Antigen (CD44) Antibody

abx413849-01mg 0.1 mg
EUR 453

CD44 Antigen (CD44) Antibody

abx414285-02mg 0.2 mg
EUR 565

CD44 Antigen (CD44) Antibody

abx414286-25ug 25 ug
EUR 272

CD44 Antigen (CD44) Antibody

abx414525-02mg 0.2 mg
EUR 565

CD44 Antigen (CD44) Antibody

abx414526-25ug 25 ug
EUR 272

CD44 Antigen (CD44) Antibody

abx414875-025mg 0.25 mg
EUR 565

CD44 Antigen (CD44) Antibody

abx415170-025mg 0.25 mg
EUR 592

CD44 Antigen (CD44) Antibody

abx415396-01mg 0.1 mg
EUR 439

CD44 Antigen (CD44) Antibody

abx415398-025mg 0.25 mg
EUR 565

CD44 Antigen (CD44) Antibody

abx415399-1mg 1 mg
EUR 829

CD44 Antigen (CD44) Antibody

abx415545-01mg 0.1 mg
EUR 453

CD44 Antigen (CD44) Antibody

abx415612-02mg 0.2 mg
EUR 565

CD44 Antigen (CD44) Antibody

abx415623-20ug 20 ug
EUR 272

CD44 Antigen (CD44) Antibody

abx432475-200ul 200 ul
EUR 384

CD44 Antigen (CD44) Antibody

abx200293-100ug 100 ug
EUR 328

CD44 Antigen (CD44) Antibody

20-abx200798
  • EUR 272.00
  • EUR 439.00
  • 100 ug
  • 500 ug

CD44 Antigen (CD44) Antibody

20-abx241364
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

CD44 Antigen (CD44) Antibody

20-abx242943
  • EUR 467.00
  • EUR 3042.00
  • EUR 328.00
  • 100 ul
  • 1 ml
  • 50 ul

CD44 Antigen (CD44) Antibody

20-abx212503
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

CD44 Antigen (CD44) Antibody

20-abx211318
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

CD44 Antigen (CD44) Antibody

abx231482-100ug 100 ug
EUR 509

CD44 Antigen (CD44) Antibody

abx231483-100ug 100 ug
EUR 481

CD44 Antigen (CD44) Antibody

abx332801-100ul 100 ul
EUR 425

CD44 Antigen (CD44) Antibody

20-abx327913
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

CD44 Antigen (CD44) Antibody (FITC)

abx134409-100Assays 100 Assays
EUR 439

CD44 Antigen (CD44) Antibody (FITC)

abx134409-50Assays 50 Assays
EUR 342

CD44 Antigen (CD44) Antibody (PE)

abx134410-100Assays 100 Assays
EUR 495

CD44 Antigen (CD44) Antibody (PE)

abx134410-50Assays 50 Assays
EUR 398

CD44 Antigen (CD44) Antibody (PE)

abx139286-01mg 0.1 mg
EUR 342

CD44 Antigen (CD44) Antibody (PE)

abx139278-100tests 100 tests
EUR 481

CD44 Antigen (CD44) Antibody (APC)

abx139279-100tests 100 tests
EUR 481

CD44 Antigen (CD44) Antibody (Biotin)

abx139280-01mg 0.1 mg
EUR 425

CD44 Antigen (CD44) Antibody (FITC)

abx139281-100tests 100 tests
EUR 425

CD44 Antigen (CD44) Antibody (PE)

abx139282-100tests 100 tests
EUR 481

CD44 Antigen (CD44) Antibody (APC)

abx139283-100tests 100 tests
EUR 481

CD44 Antigen (CD44) Antibody (FITC)

abx139284-100tests 100 tests
EUR 425

CD44 Antigen (CD44) Antibody (Biotin)

abx139288-01mg 0.1 mg
EUR 314

CD44 Antigen (CD44) Antibody (FITC)

abx412636-01mg 0.1 mg
EUR 509

CD44 Antigen (CD44) Antibody (FITC)

abx412637-05mg 0.5 mg
EUR 718

CD44 Antigen (CD44) Antibody (FITC)

abx412638-25ug 25 ug
EUR 272

CD44 Antigen (CD44) Antibody (FITC)

abx412738-01mg 0.1 mg
EUR 648

CD44 Antigen (CD44) Antibody (RPE)

abx412740-100tests 100 tests
EUR 718

CD44 Antigen (CD44) Antibody (FITC)

abx413848-01mg 0.1 mg
EUR 648

CD44 Antigen (CD44) Antibody (FITC)

abx414183-01mg 0.1 mg
EUR 648

CD44 Antigen (CD44) Antibody (FITC)

abx415394-01mg 0.1 mg
EUR 509

CD44 Antigen (CD44) Antibody (FITC)

abx415395-50ug 50 ug
EUR 342

CD44 Antigen (CD44) Antibody (RPE)

abx415397-100tests 100 tests
EUR 592

CD44 Antigen (CD44) Antibody (FITC)

abx415619-01mg 0.1 mg
EUR 509

CD44 Antigen (CD44) Antibody (FITC)

abx415620-25ug 25 ug
EUR 272

CD44 Antigen (CD44) Antibody (RPE)

abx415621-100tests 100 tests
EUR 592

CD44 Antigen (CD44) Antibody (RPE)

abx415622-25tests 25 tests
EUR 314

CD44 Antigen (CD44) Antibody (FITC)

abx200294-100tests 100 tests
EUR 398

CD44 Antigen (CD44) Antibody (PE)

abx200295-100tests 100 tests
EUR 551

CD44 Antigen (CD44) Antibody (APC)

abx200296-100tests 100 tests
EUR 453

CD44 Antigen (CD44) Antibody (PerCP)

abx200297-100tests 100 tests
EUR 718

CD44 Antigen (CD44) Antibody (Biotin)

abx200298-100ug 100 ug
EUR 384

CD44 Antigen (CD44) Antibody (APC)

20-abx200791
  • EUR 300.00
  • EUR 217.00
  • 100 ug
  • 25 ug

CD44 Antigen (CD44) Antibody (Biotin)

20-abx200792
  • EUR 398.00
  • EUR 230.00
  • 100 ug
  • 25 ug

CD44 Antigen (CD44) Antibody (FITC)

20-abx200794
  • EUR 300.00
  • EUR 217.00
  • EUR 314.00
  • 100 ug
  • 25 ug
  • 500 ug

CD44 Antigen (CD44) Antibody (PE)

20-abx200795
  • EUR 300.00
  • EUR 217.00
  • 100 ug
  • 25 ug

CD44 Antigen (CD44) Antibody (PerCP)

20-abx200796
  • EUR 551.00
  • EUR 300.00
  • 100 ug
  • 25 ug

Human CD44/ CD44 antigen ELISA Kit

E0426Hu 1 Kit
EUR 563

Human CD44 Antigen (CD44) ELISA Kit

abx253618-96tests 96 tests
EUR 668

Human CD44(CD44 antigen) ELISA Kit

EH0654 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human CD44 antigen, CD44 ELISA KIT

ELI-02342h 96 Tests
EUR 824

Dog CD44/ CD44 antigen ELISA Kit

E0019Do 1 Kit
EUR 717

Rat Cd44/ CD44 antigen ELISA Kit

E0180Ra 1 Kit
EUR 571

Mouse Cd44/ CD44 antigen ELISA Kit

E0253Mo 1 Kit
EUR 571

Cow CD44 Antigen (CD44) ELISA Kit

abx513662-96tests 96 tests
EUR 911

Dog CD44 Antigen (CD44) ELISA Kit

abx513663-96tests 96 tests
EUR 911

Rat CD44 Antigen (CD44) ELISA Kit

abx513666-96tests 96 tests
EUR 754

CD44 Antigen (CD44) Antibody (CF-Blue)

abx200299-100tests 100 tests
EUR 481

CD44 Antigen (CD44) Antibody (CF-Blue)

20-abx200793
  • EUR 425.00
  • EUR 286.00
  • 100 ug
  • 25 ug

CD44 Antigen (CD44) Antibody (PerCP-Cy5.5)

20-abx200797
  • EUR 495.00
  • EUR 300.00
  • 100 ug
  • 25 ug

Mouse CD44 Antigen (CD44) ELISA Kit

abx254787-96tests 96 tests
EUR 668

Rat CD44 Antigen (CD44) ELISA Kit

abx256320-96tests 96 tests
EUR 754

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