DHPLC/SURVEYOR nuclease: a sensitive, rapid and affordable

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long-qt-syndrome

DHPLC/SURVEYOR nuclease: a delicate, speedy and inexpensive methodology to investigate BRCA1 and BRCA2 mutations in breast most cancers households.

  •  Hereditary breast most cancers accounts for about 10% of all breast cancers and BRCA1 and BRCA2 genes have been recognized as validated susceptibility genes for this pathology. Testing for BRCA gene mutations is normally based mostly on a pre-screening strategy, such because the partial denaturation DHPLC methodology, and capillary direct sequencing.
  • Nevertheless, this strategy is time consuming because of the massive measurement of BRCA1 and BRCA2 genes. Lately, a brand new low value and time saving DHPLC protocol has been developed to investigate gene mutations by utilizing SURVEYOR(®) Nuclease digestion and DHPLC evaluation. A subset of 90 sufferers, enrolled within the Genetic Counseling Program of the Nationwide Most cancers Centre of Bari (Italy), was carried out to validate this strategy.
  • Earlier retrospective evaluation confirmed that 9/90 sufferers (10%) had been mutated in BRCA1 and BRCA2 genes and these knowledge had been confirmed by the current strategy. DNA samples underwent landing PCR and, subsequently, SURVEYOR(®) nuclease digestion. BRCA1 and BRCA2 amplicons had been divided into teams relying on amplicon measurement to permit multiamplicon digestion.
  • The product of this response had been analyzed on Transgenomic WAVE Nucleic Acid Excessive Sensitivity Fragment Evaluation System. The operator who carried out the DHPLC surveyor strategy didn’t know the sequencing outcomes at the moment. The SURVEYOR(®) Nuclease DHPLC strategy was capable of detect all alterations with a sensitivity of 95%. Moreover, so as to save time and reagents, a multiamplicon setting preparation was validated.
long-qt-syndrome
long-qt-syndrome

Recombinant TMPRSS2 Protein, Partial

E80017
  • EUR 423.50
  • EUR 660.00
  • EUR 2321.00
  • 20 ul
  • 100 ul
  • 1 ml

Recombinant Treponema pallidum p15 (partial)

7-07552 100µg Ask for price

Recombinant Treponema pallidum p15 (partial)

7-07553 500µg Ask for price

Recombinant Treponema pallidum p15 (partial)

7-07554 1000µg Ask for price

Recombinant Treponema pallidum p17 (Partial)

7-07564 100µg Ask for price

Recombinant Treponema pallidum p17 (Partial)

7-07565 500µg Ask for price

Recombinant Treponema pallidum p17 (Partial)

7-07566 1000µg Ask for price

Recombinant Treponema pallidum p47 (Partial)

7-07576 100µg Ask for price

Recombinant Treponema pallidum p47 (Partial)

7-07577 500µg Ask for price

Recombinant Treponema pallidum p47 (Partial)

7-07578 1000µg Ask for price

Recombinant Treponema pallidum TmpA (Partial)

7-07579 100µg Ask for price

Recombinant Treponema pallidum TmpA (Partial)

7-07580 500µg Ask for price

Recombinant Treponema pallidum TmpA (Partial)

7-07581 1000µg Ask for price

Recombinant (E.Coli) Treponema pallidum p17 (Partial)

RP-1442 100 ug
EUR 343.2

Recombinant (E.Coli) Treponema pallidum p47 ( partial)

RP-1444 100 ug
EUR 343.2

Recombinant (E.Coli) Treponema pallidum p15 (partial)

RP-1452 100 ug
EUR 343.2

Human Myosin 1(MYH1) ELISA kit

E01M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Myosin 1(MYH1) ELISA kit

E01M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Myosin 1(MYH1) ELISA kit

E01M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Recombinant (E.Coli) Treponema pallidum TmpA (partial)

RP-1446 100 ug
EUR 343.2

Recombinant Treponema pallidum p15 (Partial), His Tag

7-07558 100µg Ask for price

Recombinant Treponema pallidum p15 (Partial), His Tag

7-07559 500µg Ask for price

Recombinant Treponema pallidum p15 (Partial), His Tag

7-07560 1000µg Ask for price

Human MYH1/ Myosin-1 ELISA Kit

E1687Hu 1 Kit
EUR 685.2

Human MYH1(Myosin-1) ELISA Kit

EH0735 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Recombinant Cell surface-binding protein(E8L) ,partial

CSB-BP844976MHV1 20μg Ask for price
Description: Baculovirus

Recombinant Cell surface-binding protein(E8L) ,partial

CSB-MP844976MHV1 20μg Ask for price
Description: Mammalian cell

Recombinant Cell surface-binding protein(E8L) ,partial

CSB-YP844976MHV1 20μg Ask for price
Description: Yeast

Recombinant Cell surface-binding protein(E8L) ,partial

CSB-EP844976MHV1 20μg Ask for price
Description: E.coli

Recombinant Cell surface-binding protein(E8L) ,partial

CSB-EP844976MHV1-B 20μg Ask for price
Description: In Vivo Biotinylation in E.coli

Recombinant Myosin Heavy Chain 16 (MYH16)

4-RPD455Hu01
  • EUR 582.34
  • EUR 279.60
  • EUR 1853.76
  • EUR 697.92
  • EUR 1275.84
  • EUR 465.60
  • EUR 4454.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 16 expressed in: E.coli

Recombinant (E.Coli) Treponema pallidum p15 (Partial), His-tag

RP-1448 100 ug
EUR 343.2

Recombinant Novel Coronavirus Spike Glycoprotein(S), Partial

E80018
  • EUR 388.30
  • EUR 860.20
  • EUR 4888.40
  • 20 ul
  • 100 ul
  • 1 ml

Recombinant Transmembrane Protease Serine 2 Protein, Partial

E80016
  • EUR 470.80
  • EUR 753.50
  • EUR 1824.90
  • 20 ul
  • 100 ul
  • 500 ul

Recombinant Rat IL 13, Partial Protein, Untagged, E.coli-1mg

QP14019-EC-1mg 1mg
EUR 5680.8

Recombinant Rat IL 13, Partial Protein, Untagged, E.coli-2ug

QP14019-EC-2ug 2ug
EUR 186

Recombinant Rat IL 13, Partial Protein, Untagged, E.coli-10ug

QP14019-EC-10ug 10ug
EUR 241.2

Recombinant SARS-CoV-2 Spike Glycoprotein(S) (D614G), Partial

E80028
  • EUR 388.30
  • EUR 860.20
  • 20 ul
  • 100 ul

Recombinant human Myosin-11

P2913 100ug Ask for price
Description: Recombinant protein for human Myosin-11

Recombinant human Myosin-6

P1315 100ug Ask for price
Description: Recombinant protein for human Myosin-6

Recombinant human Myosin-6

P1499 100ug Ask for price
Description: Recombinant protein for human Myosin-6

Recombinant human Myosin-4

P2914 100ug Ask for price
Description: Recombinant protein for human Myosin-4

Recombinant Myosin Heavy Chain 14, Non Muscle (MYH14)

4-RPD454Hu01
  • EUR 569.44
  • EUR 276.00
  • EUR 1805.38
  • EUR 681.79
  • EUR 1243.58
  • EUR 456.00
  • EUR 4333.44
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 14, Non Muscle expressed in: E.coli

Recombinant Myosin Heavy Chain 10, Non Muscle (MYH10)

4-RPD423Hu01
  • EUR 582.34
  • EUR 279.60
  • EUR 1853.76
  • EUR 697.92
  • EUR 1275.84
  • EUR 465.60
  • EUR 4454.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 10, Non Muscle expressed in: E.coli

Recombinant other T. pallidum p15 (Partial) Protein, GST, E.coli-1mg

QP13670-GST-1mg 1mg
EUR 1513.2

Recombinant other T. pallidum p47 (Partial) Protein, His, E.coli-1mg

QP13676-1mg 1mg
EUR 1513.2

Recombinant Viral replication protein A28-like(A30L),partial,Yeast

CSB-YP848659MHV 1mg Ask for price

Rat Myosin 1(MYH1) ELISA kit

E02M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myosin 1(MYH1) ELISA kit

E02M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myosin 1(MYH1) ELISA kit

E02M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Myosin 1(MYH1) ELISA kit

E07M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Myosin 1(MYH1) ELISA kit

E07M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Myosin 1(MYH1) ELISA kit

E07M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Myosin 1(MYH1) ELISA kit

E08M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Myosin 1(MYH1) ELISA kit

E08M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Myosin 1(MYH1) ELISA kit

E08M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

4-RPD420Hu01
  • EUR 515.68
  • EUR 261.60
  • EUR 1603.78
  • EUR 614.59
  • EUR 1109.18
  • EUR 420.00
  • EUR 3829.44
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 11, Smooth Muscle expressed in: E.coli

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

4-RPD420Ra01
  • EUR 603.84
  • EUR 285.60
  • EUR 1934.40
  • EUR 724.80
  • EUR 1329.60
  • EUR 480.00
  • EUR 4656.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Myosin Heavy Chain 11, Smooth Muscle expressed in: E.coli

Goat Myosin 1(MYH1) ELISA kit

E06M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Myosin 1(MYH1) ELISA kit

E06M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Myosin 1(MYH1) ELISA kit

E06M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Recombinant other T. pallidum p15 (Partial) Protein, GST, E.coli-100ug

QP13670-GST-100ug 100ug
EUR 261.6

Recombinant other T. pallidum p15 (Partial) Protein, GST, E.coli-500ug

QP13670-GST-500ug 500ug
EUR 795.6

Recombinant other T. pallidum p17 (Partial) Protein, His, E.coli-100ug

QP13672-100ug 100ug
EUR 261.6

Recombinant other T. pallidum p17 (Partial) Protein, His, E.coli-500ug

QP13672-500ug 500ug
EUR 795.6

Recombinant other T. pallidum p47 (Partial) Protein, His, E.coli-100ug

QP13676-100ug 100ug
EUR 261.6

Recombinant other T. pallidum p47 (Partial) Protein, His, E.coli-500ug

QP13676-500ug 500ug
EUR 795.6

Mouse Myosin 1(MYH1) ELISA kit

E03M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Myosin 1(MYH1) ELISA kit

E03M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Myosin 1(MYH1) ELISA kit

E03M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Recombinant Monkeypox virus Envelope protein A28 homolog(A30L),partial

CSB-BP848659MHV 20μg Ask for price
Description: Baculovirus

Recombinant Monkeypox virus Envelope protein A28 homolog(A30L),partial

CSB-MP848659MHV 20μg Ask for price
Description: Mammalian cell

Recombinant Monkeypox virus Envelope protein A28 homolog(A30L),partial

CSB-EP848659MHV-B 20μg Ask for price
Description: In Vivo Biotinylation in E.coli

Recombinant other T. pallidum p17 (Partial) Protein, His, E.coli-1200ug

QP13672-1200ug 1200ug
EUR 1273.2

Rabbit Myosin 1(MYH1) ELISA kit

E04M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Myosin 1(MYH1) ELISA kit

E04M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Myosin 1(MYH1) ELISA kit

E04M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Myosin 1(MYH1) ELISA kit

E09M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Myosin 1(MYH1) ELISA kit

E09M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Myosin 1(MYH1) ELISA kit

E09M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Recombinant Human Myosin Light Chain 1

7-05632 2µg Ask for price

Recombinant Human Myosin Light Chain 1

7-05633 10µg Ask for price

Recombinant Human Myosin Light Chain 1

7-05634 1mg Ask for price

Recombinant other T. pallidum TmpA partial Protein, Untagged, E.coli-1mg

QP13678-1mg 1mg
EUR 1513.2

Pig MYH1/ Myosin-1 ELISA Kit

E0133Pi 1 Kit
EUR 860.4

Recombinant other T. pallidum TmpA partial Protein, Untagged, E.coli-100ug

QP13678-100ug 100ug
EUR 261.6

Recombinant other T. pallidum TmpA partial Protein, Untagged, E.coli-500ug

QP13678-500ug 500ug
EUR 795.6

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

4-RPD416Hu01
  • EUR 590.94
  • EUR 282.00
  • EUR 1886.02
  • EUR 708.67
  • EUR 1297.34
  • EUR 470.40
  • EUR 4535.04
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 4, Skeletal Muscle expressed in: E.coli

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

4-RPD416Mu01
  • EUR 614.59
  • EUR 288.00
  • EUR 1974.72
  • EUR 738.24
  • EUR 1356.48
  • EUR 487.20
  • EUR 4756.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Myosin Heavy Chain 4, Skeletal Muscle expressed in: E.coli

Bovine MYH1/ Myosin-1 ELISA Kit

E0186Bo 1 Kit
EUR 860.4

Recombinant DNA-directed RNA polymerase 132 kDa polypeptide(RPO132) ,partial

CSB-BP851803MHV 20μg Ask for price
Description: Baculovirus

Recombinant DNA-directed RNA polymerase 132 kDa polypeptide(RPO132) ,partial

CSB-MP851803MHV 20μg Ask for price
Description: Mammalian cell

Recombinant DNA-directed RNA polymerase 132 kDa polypeptide(RPO132) ,partial

CSB-YP851803MHV 20μg Ask for price
Description: Yeast

Recombinant DNA-directed RNA polymerase 132 kDa polypeptide(RPO132) ,partial

CSB-EP851803MHV 20μg Ask for price
Description: E.coli

Recombinant DNA-directed RNA polymerase 132 kDa polypeptide(RPO132) ,partial

CSB-EP851803MHV-B 20μg Ask for price
Description: In Vivo Biotinylation in E.coli

Guinea pig Myosin 1(MYH1) ELISA kit

E05M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Myosin 1(MYH1) ELISA kit

E05M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Myosin 1(MYH1) ELISA kit

E05M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

4-RPD418Hu01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 7, Cardiac Muscle, Beta expressed in: E.coli

Recombinant Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)

4-RPD419Ra01
  • EUR 625.34
  • EUR 290.40
  • EUR 2015.04
  • EUR 751.68
  • EUR 1383.36
  • EUR 494.40
  • EUR 4857.60
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Myosin Heavy Chain 6, Cardiac Muscle, Alpha expressed in: E.coli

Recombinant Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)

4-RPD419Ra02
  • EUR 625.34
  • EUR 290.40
  • EUR 2015.04
  • EUR 751.68
  • EUR 1383.36
  • EUR 494.40
  • EUR 4857.60
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Myosin Heavy Chain 6, Cardiac Muscle, Alpha expressed in: E.coli

Recombinant Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)

4-RPD419Ra03
  • EUR 625.34
  • EUR 290.40
  • EUR 2015.04
  • EUR 751.68
  • EUR 1383.36
  • EUR 494.40
  • EUR 4857.60
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Myosin Heavy Chain 6, Cardiac Muscle, Alpha expressed in: E.coli

Recombinant Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B)

4-RPP164Hu01
  • EUR 537.18
  • EUR 267.60
  • EUR 1684.42
  • EUR 641.47
  • EUR 1162.94
  • EUR 434.40
  • EUR 4031.04
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 7B, Cardiac Muscle Beta expressed in: E.coli

Genorise® recombinant human myosin 1 protein

GR119100 5 µg
EUR 285

ELISA kit for Canine Myosin-1 (MYH1)

KTE20052-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Canine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Canine Myosin-1 (MYH1)

KTE20052-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Canine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Canine Myosin-1 (MYH1)

KTE20052-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Canine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Myosin-1 (MYH1)

KTE10236-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Bovine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Myosin-1 (MYH1)

KTE10236-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Bovine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Myosin-1 (MYH1)

KTE10236-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Bovine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Recombinant Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2)

4-RPA755Hu01
  • EUR 556.54
  • EUR 272.40
  • EUR 1756.99
  • EUR 665.66
  • EUR 1211.33
  • EUR 447.60
  • EUR 4212.48
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 2, Skeletal Muscle, Adult expressed in: Prokaryotic expression

Recombinant Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2)

4-RPA755Mu01
  • EUR 595.24
  • EUR 283.20
  • EUR 1902.14
  • EUR 714.05
  • EUR 1308.10
  • EUR 474.00
  • EUR 4575.36
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Myosin Heavy Chain 2, Skeletal Muscle, Adult expressed in: E.coli

Recombinant Salmonella OMPA Protein (Partial of the Full Length of 22-350aa, 27-349aa)

VAng-Wyb0566-10g 10 µg
EUR 930
Description: Salmonella Outer membrane protein A, recombinant protein.

Recombinant Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3)

4-RPD415Hu01
  • EUR 603.84
  • EUR 285.60
  • EUR 1934.40
  • EUR 724.80
  • EUR 1329.60
  • EUR 480.00
  • EUR 4656.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 3, Skeletal Muscle, Embryonic expressed in: E.coli

Recombinant Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3)

4-RPD415Ra01
  • EUR 599.54
  • EUR 283.20
  • EUR 1918.27
  • EUR 719.42
  • EUR 1318.85
  • EUR 476.40
  • EUR 4615.68
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Myosin Heavy Chain 3, Skeletal Muscle, Embryonic expressed in: E.coli

Recombinant Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8)

4-RPD421Ra01
  • EUR 575.88
  • EUR 278.40
  • EUR 1829.57
  • EUR 689.86
  • EUR 1259.71
  • EUR 460.80
  • EUR 4393.92
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Myosin Heavy Chain 8, Skeletal Muscle, Perinatal expressed in: E.coli

Recombinant Human Myosin Light Chain 2

7-05635 2µg Ask for price

Recombinant Human Myosin Light Chain 2

7-05636 25µg Ask for price

Recombinant Human Myosin Light Chain 2

7-05637 1mg Ask for price

Recombinant Human Myosin Light Chain 6

7-05638 2µg Ask for price

Recombinant Human Myosin Light Chain 6

7-05639 10µg Ask for price

Recombinant Human Myosin Light Chain 6

7-05640 1mg Ask for price

Recombinant Human Myosin Light Chain 9

7-05641 2µg Ask for price

Recombinant Human Myosin Light Chain 9

7-05642 10µg Ask for price

Recombinant Human Myosin Light Chain 9

7-05643 1mg Ask for price

Recombinant Human Myosin Light Chain 12A

7-05644 5µg Ask for price

Recombinant Human Myosin Light Chain 12A

7-05645 20µg Ask for price

Recombinant Human Myosin Light Chain 12A

7-05646 1mg Ask for price

Recombinant FMD serotype SAT-1, partial viral protein (FMDS-VP1) protein control for western blot

FMDS11-C 100 ul
EUR 343.2

MYL1 Myosin Light Chain 1 Human Recombinant Protein

PROTP05976 Regular: 10ug
EUR 380.4
Description: Recombinant Human Ventricular Myosin Light Chain-1 (MYL1) protein has a molecular mass of 25 kDa and is fused to 7 amino acids at N-terminus. The MYL1 protein was affinity purified using anti MYL1 monoclonal antibody 39-15 column.

Recombinant Human Myosin-9 Protein, GST, E.coli-1mg

QP8366-ec-1mg 1mg
EUR 1958.4

Genorise® recombinant human myosin 2 protein

GR119101 5 µg
EUR 285

Genorise® recombinant human myosin 3 protein

GR119102 5 µg
EUR 285

Genorise® recombinant human myosin 4 protein

GR119103 5 µg
EUR 285

Genorise® recombinant human myosin 5 protein

GR119104 5 µg
EUR 285

Genorise® recombinant human myosin 6 protein

GR119105 5 µg
EUR 285

Genorise® recombinant human myosin 7 protein

GR119106 5 µg
EUR 285

Genorise® recombinant human myosin 8 protein

GR119107 5 µg
EUR 285

Genorise® recombinant human myosin 9 protein

GR119108 5 µg
EUR 285

Genorise® recombinant human myosin 10 protein

GR119109 5 µg
EUR 285

Genorise® recombinant human myosin 11 protein

GR119110 5 µg
EUR 285

Recombinant Human Myosin-9 Protein, GST, E.coli-10ug

QP8366-ec-10ug 10ug
EUR 240

Recombinant Human Myosin-9 Protein, GST, E.coli-50ug

QP8366-ec-50ug 50ug
EUR 315.6

Recombinant Human Myosin-6 Protein, His, Yeast-1mg

QP9299-ye-1mg 1mg
EUR 2262

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Protein

20-abx168162
  • EUR 811.20
  • EUR 326.40
  • EUR 2481.60
  • EUR 961.20
  • EUR 577.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Recombinant Human Myosin-9 Protein, GST, E.coli-100ug

QP8366-ec-100ug 100ug
EUR 489.6

Recombinant Human Myosin-9 Protein, GST, E.coli-200ug

QP8366-ec-200ug 200ug
EUR 760.8

Recombinant Human Myosin-9 Protein, GST, E.coli-500ug

QP8366-ec-500ug 500ug
EUR 1272

Recombinant Human Myosin-6 Protein, His, Yeast-10ug

QP9299-ye-10ug 10ug
EUR 283.2

Recombinant Human Myosin-6 Protein, His, Yeast-50ug

QP9299-ye-50ug 50ug
EUR 358.8

Recombinant Human Myosin-6 Protein, His, Yeast-100ug

QP9299-ye-100ug 100ug
EUR 576

Recombinant Human Myosin-6 Protein, His, Yeast-200ug

QP9299-ye-200ug 200ug
EUR 892.8

Recombinant Human Myosin-6 Protein, His, Yeast-500ug

QP9299-ye-500ug 500ug
EUR 1447.2

Recombinant Myosin IE (MYO1E)

4-RPD434Mu01
  • EUR 588.79
  • EUR 280.80
  • EUR 1877.95
  • EUR 705.98
  • EUR 1291.97
  • EUR 469.20
  • EUR 4514.88
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Myosin IE expressed in: E.coli

Recombinant Myosin IF (MYO1F)

4-RPD435Hu01
  • EUR 575.88
  • EUR 278.40
  • EUR 1829.57
  • EUR 689.86
  • EUR 1259.71
  • EUR 460.80
  • EUR 4393.92
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin IF expressed in: E.coli

Recombinant Myosin IF (MYO1F)

4-RPD435Ra01
  • EUR 612.44
  • EUR 286.80
  • EUR 1966.66
  • EUR 735.55
  • EUR 1351.10
  • EUR 484.80
  • EUR 4736.64
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Myosin IF expressed in: E.coli

Recombinant Myosin IG (MYO1G)

4-RPD436Hu01
  • EUR 623.20
  • EUR 290.40
  • EUR 2006.98
  • EUR 748.99
  • EUR 1377.98
  • EUR 492.00
  • EUR 4837.44
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin IG expressed in: E.coli

Recombinant Myosin IG (MYO1G)

4-RPD436Mu01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Myosin IG expressed in: E.coli

Recombinant Myosin VA (MYO5A)

4-RPD424Hu01
  • EUR 582.34
  • EUR 279.60
  • EUR 1853.76
  • EUR 697.92
  • EUR 1275.84
  • EUR 465.60
  • EUR 4454.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin VA expressed in: E.coli

Recombinant Myosin VA (MYO5A)

4-RPD424Ra01
  • EUR 512.45
  • EUR 260.40
  • EUR 1591.68
  • EUR 610.56
  • EUR 1101.12
  • EUR 418.80
  • EUR 3799.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Myosin VA expressed in: E.coli

Recombinant Myosin IB (MYO1B)

4-RPD431Mu01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Myosin IB expressed in: E.coli

Recombinant Myosin IB (MYO1B)

4-RPD431Ra01
  • EUR 614.59
  • EUR 288.00
  • EUR 1974.72
  • EUR 738.24
  • EUR 1356.48
  • EUR 487.20
  • EUR 4756.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Myosin IB expressed in: E.coli

Recombinant Myosin IC (MYO1C)

4-RPD432Hu01
  • EUR 580.19
  • EUR 278.40
  • EUR 1845.70
  • EUR 695.23
  • EUR 1270.46
  • EUR 463.20
  • EUR 4434.24
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin IC expressed in: E.coli

Recombinant Myosin IC (MYO1C)

4-RPD432Ra01
  • EUR 601.69
  • EUR 284.40
  • EUR 1926.34
  • EUR 722.11
  • EUR 1324.22
  • EUR 477.60
  • EUR 4635.84
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Myosin IC expressed in: E.coli

Recombinant Myosin ID (MYO1D)

4-RPD433Mu01
  • EUR 603.84
  • EUR 285.60
  • EUR 1934.40
  • EUR 724.80
  • EUR 1329.60
  • EUR 480.00
  • EUR 4656.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Myosin ID expressed in: E.coli

Recombinant Myosin ID (MYO1D)

4-RPD433Ra01
  • EUR 633.95
  • EUR 292.80
  • EUR 2047.30
  • EUR 762.43
  • EUR 1404.86
  • EUR 499.20
  • EUR 4938.24
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Myosin ID expressed in: E.coli

Recombinant Myosin IE (MYO1E)

4-RPD434Hu01
  • EUR 560.83
  • EUR 273.60
  • EUR 1773.12
  • EUR 671.04
  • EUR 1222.08
  • EUR 451.20
  • EUR 4252.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin IE expressed in: E.coli

Recombinant Myosin IA (MYO1A)

4-RPA375Hu01
  • EUR 571.58
  • EUR 276.00
  • EUR 1813.44
  • EUR 684.48
  • EUR 1248.96
  • EUR 458.40
  • EUR 4353.60
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin IA expressed in: E.coli

Recombinant Myosin IA (MYO1A)

4-RPA375Mu01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Myosin IA expressed in: E.coli

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) CLIA Kit

20-abx491987
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Myosin heavy polypeptide 1 (MYH1) polyclonal antibody

ABP-PAB-01616 100 ug Ask for price

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

abx053857-96tests 96 tests
EUR 904.8

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

DLR-MYH1-Hu-48T 48T
EUR 574.8
Description: A sandwich quantitative ELISA assay kit for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

DLR-MYH1-Hu-96T 96T
EUR 745.2
Description: A sandwich quantitative ELISA assay kit for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

abx253693-96tests 96 tests
EUR 904.8

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

20-abx152417
  • EUR 7970.40
  • EUR 4250.40
  • EUR 990.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

abx570236-96tests 96 tests
EUR 801.6

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult(MYH1)ELISA Kit

QY-E03032 96T
EUR 433.2

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

SEA754Hu-10x96wellstestplate 10x96-wells test plate
EUR 5128.02
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

SEA754Hu-1x48wellstestplate 1x48-wells test plate
EUR 527.48
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

SEA754Hu-1x96wellstestplate 1x96-wells test plate
EUR 702.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

SEA754Hu-5x96wellstestplate 5x96-wells test plate
EUR 2799.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

4-SEA754Hu
  • EUR 5188.80
  • EUR 2739.60
  • EUR 703.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

RD-MYH1-Hu-48Tests 48 Tests
EUR 573.6

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

RD-MYH1-Hu-96Tests 96 Tests
EUR 794.4

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

RDR-MYH1-Hu-48Tests 48 Tests
EUR 600

Identification of copy quantity variation of CAPN10 in Thais with kind 2 diabetes by multiplex PCR and denaturing excessive efficiency liquid chromatography (DHPLC).

 Copy quantity variations (CNVs) have been proven to be related to a number of illnesses. They will trigger deviation of genotypes from Hardy-Weinberg Equilibrium (HWE). Genetic case-control affiliation research in Thais revealed that genotype distribution of CAPN10 Indel19 was deviated from HWE after correction of genotyping error. Due to this fact, we goal to establish CNVs inside CAPN10 Indel19 area.

The semi-quantitative denaturating excessive efficiency liquid chromatography (DHPLC) methodology was used to detect CNVs within the area of CAPN10 Indel19 marker in cohort of 305 sufferers with kind 2 diabetes and 250 management topics with out diabetes. CNVs within the area of CAPN10 Indel19 was efficiently detected by DHPLC.

After correction of genotype calling based mostly on the standing of recognized CNVs, CAPN10 Indel19 genotypes had been well-fitted for HWE (p>0.05). Nevertheless, we didn’t discover affiliation between CNV genotypes and threat of kind 2 diabetes in our inhabitants. CNVs in CAPN10 have been recognized in Thais. These CNVs result in deviation from HWE of CAPN10 Indel19 genotypes. After excluding recognized CNVs from the evaluation, CAPN10 Indel19 was related to kind 2 diabetes. The data obtained from our research could be useful for genotyping accuracies of SNPs residing within the CNVs area.

 

Excessive-performance liquid chromatography underneath partially denaturing situations (dHPLC) is a quick and cost-effective methodology for screening molecular defects: 4 novel mutations present in X-linked continual granulomatous illness.

 Implementing exact methods in routine analysis of continual granulomatous illness (CGD), which expedite the screening of molecular defects, could also be important for a fast assumption of affected person prognosis. This research in contrast the efficacy of single-strand conformation polymorphism evaluation (SSCP) and high-performance liquid chromatography underneath partially denaturing situations (dHPLC) for screening mutations in CGD sufferers. We chosen 10 male CGD sufferers with a scientific historical past of extreme recurrent infections and irregular respiratory burst operate. gDNA, mRNA and cDNA samples had been ready by commonplace strategies. CYBB exons had been amplified by PCR and screened by SSCP or dHPLC. Irregular DNA fragments had been sequenced to disclose the character of the mutations.

The SSCP and dHPLC strategies confirmed DNA abnormalities, respectively, in 55% and 100% of the circumstances. Sequencing of the irregular DNA samples confirmed mutations in all circumstances. 4 novel mutations in CYBB had been recognized which had been picked up solely by the dHPLC screening (c.904 insC, c.141+5 g>t, c.553 T>C, and c.665 A>T).

 

This work highlights the relevance of dHPLC, a delicate, quick, dependable and cost-effective methodology for screening mutations in CGD, which in mixture with practical assays assessing the phagocyte respiratory burst will contribute to expedite the definitive analysis of X-linked CGD, direct therapy, genetic counselling and to have a transparent assumption of the prognosis. This technique is very appropriate for creating international locations.

 

dHPLC effectivity for semi-automated cDNA-AFLP analyses and fragment assortment within the apple scab-resistance gene mannequin.

  •  cDNA-AFLP evaluation for transcript profiling has been efficiently utilized to review many plant organic methods, notably plant-microbe interactions. Nevertheless, the separation of cDNA-AFLP fragments by gel electrophoresis is reported to be labor-intensive with solely restricted potential for automation, and the gathering of differential bands for gene identification is much more cumbersome.
  • On this work, we current using dHPLC (denaturing excessive efficiency liquid chromatography) and automatic DNA fragment assortment utilizing the WAVE(®) System to investigate and get better cDNA-AFLP fragments.
  • The tactic is efficiently utilized to the Malus-Venturia inaequalis interplay, making it attainable to gather 66 totally different transcript-derived fragments for apple genes putatively concerned within the protection response activated by the HcrVf2 resistance gene.
  • The outcomes, validated by actual time quantitative RT-PCR, had been according to the plant-pathogen interplay underneath investigation and this additional helps the suitability of dHPLC for cDNA-AFLP transcript profiling.
  • Options and benefits of this new strategy are mentioned, evincing that it’s an nearly absolutely automatable and cost-effective answer for processing massive numbers of samples and amassing differential genes concerned in different organic processes and non-model crops.

Using DHPLC (Denaturing Excessive Efficiency Liquid Chromatography) in II degree screening of the CFTR gene in Prenatal Prognosis.

 OBJECTIVE

The goal of the research is to guage the function of Denaturing Excessive Efficiency Liquid Chromatography (DHPLC) within the second degree screening of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene.

METHODS

A 9-month potential research, between June 2008 and March 2009 at Artemisia Fetal Medical Centre, included 3829 samples of amniotic fluid collected from girls present process mid-trimester amniocentesis.The genetic analysis of CF was based mostly on analysis of the primary mutations of the CFTR gene on fetal DNA extracted from the amniocytes, (first degree screening) utilizing totally different industrial diagnostic methods. A second degree screening utilizing DHPLC, on the amniotic fluid and on a blood pattern from the couple, was provided in case of fetuses heterozygous at first degree screening.

RESULTS

Of 3829 fetuses, 134 had been discovered to be constructive, 129 heterozygous and 5 affected. Of the 129 {couples}, following acceptable genetic counselling, 53 requested a second degree screening. Via using DHPLC, 44 {couples} had been discovered to be destructive, and in 9 {couples}, 9 uncommon mutations had been recognized.

CONCLUSIONS

The primary degree screening will be helpful to proof as much as 75% of the CF mutations. The second degree screening can establish an extra 10% of mutant alleles. DHPLC was discovered to be a dependable and particular methodology for the speedy identification of the uncommon CFTR mutations which weren’t revealed in preliminary first degree screening.

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