DHPLC/SURVEYOR nuclease: a sensitive, rapid and affordable

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long-qt-syndrome

DHPLC/SURVEYOR nuclease: a delicate, speedy and inexpensive methodology to investigate BRCA1 and BRCA2 mutations in breast most cancers households.

  •  Hereditary breast most cancers accounts for about 10% of all breast cancers and BRCA1 and BRCA2 genes have been recognized as validated susceptibility genes for this pathology. Testing for BRCA gene mutations is normally based mostly on a pre-screening strategy, such because the partial denaturation DHPLC methodology, and capillary direct sequencing.
  • Nevertheless, this strategy is time consuming because of the massive measurement of BRCA1 and BRCA2 genes. Lately, a brand new low value and time saving DHPLC protocol has been developed to investigate gene mutations by utilizing SURVEYOR(®) Nuclease digestion and DHPLC evaluation. A subset of 90 sufferers, enrolled within the Genetic Counseling Program of the Nationwide Most cancers Centre of Bari (Italy), was carried out to validate this strategy.
  • Earlier retrospective evaluation confirmed that 9/90 sufferers (10%) had been mutated in BRCA1 and BRCA2 genes and these knowledge had been confirmed by the current strategy. DNA samples underwent landing PCR and, subsequently, SURVEYOR(®) nuclease digestion. BRCA1 and BRCA2 amplicons had been divided into teams relying on amplicon measurement to permit multiamplicon digestion.
  • The product of this response had been analyzed on Transgenomic WAVE Nucleic Acid Excessive Sensitivity Fragment Evaluation System. The operator who carried out the DHPLC surveyor strategy didn’t know the sequencing outcomes at the moment. The SURVEYOR(®) Nuclease DHPLC strategy was capable of detect all alterations with a sensitivity of 95%. Moreover, so as to save time and reagents, a multiamplicon setting preparation was validated.
long-qt-syndrome
long-qt-syndrome

Recombinant Human Myosin-9(MYH9) ,partial

AP71716 1mg
EUR 1978

Recombinant Human Myosin-6(MYH6),partial

AP72695 each Ask for price

Recombinant Human Myosin-6 (MYH6), partial

CSB-EP015299HU 6810 mg Ask for price

Recombinant Human Myosin-6 (MYH6), partial

CSB-EP015299HUa0 9932 mg Ask for price

Recombinant Human Myosin-7 (MYH7), partial

CSB-EP015300HU 9938 mg Ask for price

Recombinant Human Myosin-9 (MYH9), partial

CSB-EP015303HUe0 1758 mg Ask for price

Recombinant Human Myosin-6 (MYH6), partial

CSB-BP015299HU 10121 mg Ask for price

Recombinant Human Myosin-7 (MYH7), partial

CSB-BP015300HU 9781 mg Ask for price

Recombinant Human Myosin-9 (MYH9) ,partial

RPC20174-100ug 100ug
EUR 460.9

Recombinant Human Myosin-9 (MYH9) ,partial

RPC20174-1mg 1mg
EUR 2237.8

Recombinant Human Myosin-9 (MYH9) ,partial

RPC20174-20ug 20ug
EUR 258.7

Recombinant Human Myosin-6 (MYH6) ,partial

RPC20235-100ug 100ug
EUR 573.1

Recombinant Human Myosin-6 (MYH6) ,partial

RPC20235-20ug 20ug
EUR 288.8

Recombinant Rat Myosin-9 (Myh9), partial

CSB-EP717285RA 6891 mg Ask for price

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU56815-100ug 100ug
EUR 470.4

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU56815-1mg 1mg
EUR 2184

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU56815-50ug 50ug
EUR 385

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU52400-100ug 100ug
EUR 524.7

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU52400-1mg 1mg
EUR 2325.7

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU52400-50ug 50ug
EUR 421.3

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU42866-100ug 100ug
EUR 554.4

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU42866-1mg 1mg
EUR 2457

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU42866-50ug 50ug
EUR 445.5

Recombinant Myosin Heavy Chain 1 (MYH1)

RPA754Hu01 10ug
EUR 170

Recombinant Myosin Heavy Chain 1 (MYH1)

RPA754Mu01 10ug
EUR 180

Recombinant Human Unconventional myosin-X (MYO10), partial

CSB-EP884628HU 11647 mg Ask for price

Recombinant Human Unconventional myosin-VIIa(MYO7A),partial

AP74492 1mg
EUR 2826

Recombinant Human Unconventional myosin-VIIa (MYO7A), partial

CSB-EP622657HU 3794 mg Ask for price

Recombinant Human Myosin light polypeptide 6 (MYL6), partial

CSB-RP051544h 5190 mg Ask for price

Recombinant Human Myosin light polypeptide 6(MYL6),partial

AP71873 1mg
EUR 1978

Recombinant Human Myosin light chain kinase, smooth muscle (MYLK), partial

CSB-EP618995HU 6898 mg Ask for price

Recombinant Human Myosin light chain kinase, smooth muscle (MYLK) ,partial

RPC28370-100ug 100ug
EUR 635.8

Recombinant Human Myosin light chain kinase, smooth muscle (MYLK) ,partial

RPC28370-1mg 1mg
EUR 2885.2

Recombinant Human Myosin light chain kinase, smooth muscle (MYLK) ,partial

RPC28370-20ug 20ug
EUR 353.4

Recombinant Human Myosin-binding protein C, fast-type (MYBPC2),partial

CSB-YP618884HU 6490 mg Ask for price

Recombinant Human Myosin-binding protein C, fast-type(MYBPC2),partial

AP73044 each Ask for price

Recombinant Human Myosin-binding protein C, fast-type(MYBPC2),partial

AP74455 1mg
EUR 1978

Recombinant Human Myosin-binding protein C, fast-type (MYBPC2),partial

CSB-EP618884HU 3754 mg Ask for price

Recombinant Human Myosin-binding protein C, cardiac-type(MYBPC3),partial

AP74047 1mg
EUR 1978

Recombinant Human Myosin-binding protein C, cardiac-type (MYBPC3),partial

CSB-EP613524HU 3702 mg Ask for price

Recombinant Human Myosin-binding protein C, cardiac-type (MYBPC3) ,partial

RPC20381-100ug 100ug
EUR 460.9

Recombinant Human Myosin-binding protein C, cardiac-type (MYBPC3) ,partial

RPC20381-1mg 1mg
EUR 2237.8

Recombinant Human Myosin-binding protein C, cardiac-type (MYBPC3) ,partial

RPC20381-20ug 20ug
EUR 258.7

Recombinant Penaeus monodon Myosin light chain, partial

CSB-EP310401ETF 11493 mg Ask for price

Recombinant Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)

4-RPA754Hu01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 1, Skeletal Muscle, Adult expressed in: E.coli

Recombinant Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)

4-RPA754Mu01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult expressed in: E.coli

Recombinant Chicken Myosin light chain kinase, smooth muscle(Mylk),partial

AP77225 1mg
EUR 2826

Recombinant Chicken Myosin light chain kinase, smooth muscle (Mylk),partial

CSB-EP320904CHb3 2815 mg Ask for price

Recombinant Chicken Myosin light chain kinase, smooth muscle (Mylk) ,partial

RPC22429-100ug 100ug
EUR 721.6

Recombinant Chicken Myosin light chain kinase, smooth muscle (Mylk) ,partial

RPC22429-1mg 1mg
EUR 2884.7

Recombinant Chicken Myosin light chain kinase, smooth muscle (Mylk) ,partial

RPC22429-20ug 20ug
EUR 448.1

Recombinant Human Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MYL2),partial

CSB-RP100574h 5280 mg Ask for price

Recombinant Human Myosin regulatory light chain 2, ventricular/cardiac muscle isoform(MYL2),partial

AP72101 1mg
EUR 1978

Recombinant Rat Myosin-binding protein C, cardiac-type (Mybpc3),partial

CSB-YP015267RA 5859 mg Ask for price

Recombinant Rat Myosin-binding protein C, cardiac-type(Mybpc3),partial

AP72693 1mg
EUR 2834

Human Myosin-1 (MYH1) Protein

abx168162-1ml 1 ml
EUR 225

Human Myosin 1(MYH1) ELISA kit

E01A5966 96T
EUR 700
Description: ELISA

Human Myosin 1(MYH1) ELISA kit

E01M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Myosin 1(MYH1) ELISA kit

E01M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Myosin 1(MYH1) ELISA kit

E01M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Recombinant Myosin Heavy Chain 16 (MYH16)

RPD455Hu01 10ug
EUR 172

Recombinant Myosin Heavy Chain 16 (MYH16)

4-RPD455Hu01
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  • Ask for price
  • Ask for price
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 16 expressed in: E.coli

Recombinant Myosin Heavy Chain 16 (MYH16)

RPU42869-100ug 100ug
EUR 530.2

Recombinant Myosin Heavy Chain 16 (MYH16)

RPU42869-1mg 1mg
EUR 2347.8

Recombinant Myosin Heavy Chain 16 (MYH16)

RPU42869-50ug 50ug
EUR 425.7

Human MYH1/ Myosin-1 ELISA Kit

E1687Hu 1 Kit
EUR 515
Description: Myosin heavy chain 1,Myosin heavy chain 2x,Myosin heavy chain IIx/d,Myosin heavy chain, skeletal muscle, adult 1,MyHC-2x,MyHC-IIx/d

Human MYH1(Myosin-1) ELISA Kit

EH0735 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Recombinant Myosin Heavy Chain 2 (MYH2)

RPU56816-100ug 100ug
EUR 470.4

Recombinant Myosin Heavy Chain 2 (MYH2)

RPU56816-1mg 1mg
EUR 2184

Recombinant Myosin Heavy Chain 2 (MYH2)

RPU56816-50ug 50ug
EUR 385

Recombinant Myosin Heavy Chain 2 (MYH2)

RPU55243-100ug 100ug
EUR 500.5

Recombinant Myosin Heavy Chain 2 (MYH2)

RPU55243-1mg 1mg
EUR 2216.5

Recombinant Myosin Heavy Chain 2 (MYH2)

RPU55243-50ug 50ug
EUR 401.5

Recombinant Myosin Heavy Chain 2 (MYH2)

RPU42870-100ug 100ug
EUR 544.5

Recombinant Myosin Heavy Chain 2 (MYH2)

RPU42870-1mg 1mg
EUR 2412.8

Recombinant Myosin Heavy Chain 2 (MYH2)

RPU42870-50ug 50ug
EUR 437.8

Recombinant Myosin Heavy Chain 2 (MYH2)

RPA755Hu01 10ug
EUR 162

Recombinant Myosin Heavy Chain 2 (MYH2)

RPA755Mu01 10ug
EUR 177

Myosin-1 (MYH1) Antibody

abx005265-100l 100 µl
EUR 400

Myosin-1 (MYH1) Antibody

abx005265-20l 20 µl
EUR 175

Myosin-1 (MYH1) Antibody

abx005265-50l 50 µl
EUR 275

Myosin-1 (MYH1) Antibody

abx104339-100g 100 µg
EUR 687.5

Myosin-1 (MYH1) Antibody

abx104339-20g 20 µg
EUR 262.5

Myosin-1 (MYH1) Antibody

abx104339-50g 50 µg
EUR 325

Myosin-1 (MYH1) Antibody

abx128730-1096tests 10 × 96 tests
EUR 312.5

Myosin-1 (MYH1) Antibody

abx128730-596tests 5 × 96 tests
EUR 250

Myosin-1 (MYH1) Antibody

abx173673-100l 100 µl
EUR 712.5

Myosin-1 (MYH1) Antibody

abx173673-1ml 1 ml Ask for price

Myosin-1 (MYH1) Antibody

abx173673-200l 200 µl Ask for price

Myosin-1 (MYH1) Antibody

abx212233-100l 100 µl
EUR 350

Myosin-1 (MYH1) Antibody

abx212233-50l 50 µl
EUR 250

Myosin-1 (MYH1) Antibody

abx235469-100g 100 µg
EUR 350

Myosin-1 (MYH1) Antibody

abx235470-100g 100 µg
EUR 350

Myosin-1 (MYH1) Antibody

abx177671-96tests 96 tests
EUR 262.5

Myosin-1 (MYH1) Antibody

abx212641-100l 100 µl
EUR 350

Myosin-1 (MYH1) Antibody

abx212641-50l 50 µl
EUR 250

Myosin-1 (MYH1) Antibody

abx376285-96tests 96 tests
EUR 337.5

Myosin-1 (MYH1) Antibody

abx320249-100l 100 µl
EUR 350

Myosin-1 (MYH1) Antibody

abx320249-50l 50 µl
EUR 237.5

Recombinant human Myosin-11

P2913 100ug Ask for price
Description: Recombinant protein for human Myosin-11

Recombinant human Myosin-4

P2914 100ug Ask for price
Description: Recombinant protein for human Myosin-4

Recombinant human Myosin-6

P1499 100ug Ask for price
Description: Recombinant protein for human Myosin-6

Recombinant human Myosin-6

P1315 100ug Ask for price
Description: Recombinant protein for human Myosin-6

Recombinant Myosin Heavy Chain 10, Non Muscle (MYH10)

RPU52670-100ug 100ug
EUR 530.2

Recombinant Myosin Heavy Chain 10, Non Muscle (MYH10)

RPU52670-1mg 1mg
EUR 2347.8

Recombinant Myosin Heavy Chain 10, Non Muscle (MYH10)

RPU52670-50ug 50ug
EUR 425.7

Recombinant Myosin Heavy Chain 10, Non Muscle (MYH10)

RPD423Hu01 10ug
EUR 172

Recombinant Myosin Heavy Chain 10, Non Muscle (MYH10)

4-RPD423Hu01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 10, Non Muscle expressed in: E.coli

Recombinant Myosin Heavy Chain 14, Non Muscle (MYH14)

RPD454Hu01 10ug
EUR 167

Recombinant Myosin Heavy Chain 14, Non Muscle (MYH14)

4-RPD454Hu01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 14, Non Muscle expressed in: E.coli

Recombinant Myosin Heavy Chain 14, Non Muscle (MYH14)

RPU42868-100ug 100ug
EUR 514.8

Recombinant Myosin Heavy Chain 14, Non Muscle (MYH14)

RPU42868-1mg 1mg
EUR 2282.8

Recombinant Myosin Heavy Chain 14, Non Muscle (MYH14)

RPU42868-50ug 50ug
EUR 413.6

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

RPU57210-100ug 100ug
EUR 470.4

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

RPU57210-1mg 1mg
EUR 2184

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

RPU57210-50ug 50ug
EUR 385

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

RPU51044-100ug 100ug
EUR 453.2

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

RPU51044-1mg 1mg
EUR 2009.8

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

RPU51044-50ug 50ug
EUR 364.1

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

RPD420Hu01 10ug
EUR 147

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

4-RPD420Hu01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 11, Smooth Muscle expressed in: E.coli

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

RPD420Ra01 10ug
EUR 180

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

4-RPD420Ra01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Myosin Heavy Chain 11, Smooth Muscle expressed in: E.coli

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

RPD420Ra02 10ug
EUR 140

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

RPU42867-100ug 100ug
EUR 554.4

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

RPU42867-1mg 1mg
EUR 2457

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

RPU42867-50ug 50ug
EUR 445.5

Recombinant Human Myosin Light Chain 1

7-05632 2µg Ask for price

Recombinant Human Myosin Light Chain 1

7-05633 10µg Ask for price

Recombinant Human Myosin Light Chain 1

7-05634 1mg Ask for price

Recombinant Myosin Heavy Chain 9, Non Muscle (MYH9)

RPU57212-100ug 100ug
EUR 470.4

Recombinant Myosin Heavy Chain 9, Non Muscle (MYH9)

RPU57212-1mg 1mg
EUR 2184

Recombinant Myosin Heavy Chain 9, Non Muscle (MYH9)

RPU57212-50ug 50ug
EUR 385

Recombinant Myosin Heavy Chain 9, Non Muscle (MYH9)

RPD422Hu01 10ug
EUR 140

Mouse Myosin-1 (MYH1) Protein

abx068140-10g 10 µg
EUR 587.5

Mouse Myosin-1 (MYH1) Protein

abx068140-2g 2 µg
EUR 225

Mouse Myosin-1 (MYH1) Protein

abx068140-5g 5 µg
EUR 400

Myosin-1 (MYH1) Antibody (FITC)

abx271252-1ml 1 ml
EUR 975

Myosin-1 (MYH1) Antibody (FITC)

abx271252-200l 200 µl
EUR 412.5

Myosin-1 (MYH1) Antibody Pair

abx370650-100g 100 µg
EUR 2200

Myosin-1 (MYH1) Antibody Pair

abx370650-50g 50 µg
EUR 1350

Myosin-1 (MYH1) Antibody (Biotin)

abx271518-1ml 1 ml
EUR 762.5

Myosin-1 (MYH1) Antibody (Biotin)

abx271518-200l 200 µl
EUR 337.5

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

RPU57208-100ug 100ug
EUR 470.4

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

RPU57208-1mg 1mg
EUR 2184

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

RPU57208-50ug 50ug
EUR 385

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

RPD416Hu01 10ug
EUR 175

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

4-RPD416Hu01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 4, Skeletal Muscle expressed in: E.coli

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

RPD416Mu01 10ug
EUR 184

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

4-RPD416Mu01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Myosin Heavy Chain 4, Skeletal Muscle expressed in: E.coli

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

RPU42873-100ug 100ug
EUR 566.5

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

RPU42873-1mg 1mg
EUR 2511.6

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

RPU42873-50ug 50ug
EUR 455.4

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

RPU42874-100ug 100ug
EUR 540.1

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

RPU42874-1mg 1mg
EUR 2392

Recombinant Myosin Heavy Chain 4, Skeletal Muscle (MYH4)

RPU42874-50ug 50ug
EUR 433.4

Myosin Light Chain 1 Human, Recombinant

22060755-1 2 µg
EUR 137.27

Myosin Light Chain 1 Human Recombinant

rAP-3677 Inquiry Ask for price

MYH1 (untagged)-Human myosin, heavy chain 1, skeletal muscle, adult (MYH1)

SC303712 10 µg Ask for price

Human Myosin Heavy Chain 1 (MYH1) ELISA Kit

EKN47198-48T 48T
EUR 341.04

Human Myosin Heavy Chain 1 (MYH1) ELISA Kit

EKN47198-5x96T 5x96T
EUR 2314.2

Human Myosin Heavy Chain 1 (MYH1) ELISA Kit

EKN47198-96T 96T
EUR 487.2

Human Myosin Heavy Chain 1 (MYH1) ELISA Kit

EKU06087-48T 48T
EUR 500.5

Human Myosin Heavy Chain 1 (MYH1) ELISA Kit

EKU06087-5x96T 5x96T
EUR 3396.25

Human Myosin Heavy Chain 1 (MYH1) ELISA Kit

EKU06087-96T 96T
EUR 715

Human Myosin Heavy Chain 1(MYH1) ELISA Kit

NSL0420Hu 96 wells
EUR 468

Rat Myosin 1(MYH1) ELISA kit

E02M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myosin 1(MYH1) ELISA kit

E02M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myosin 1(MYH1) ELISA kit

E02M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Myosin 1(MYH1) ELISA kit

E07M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Myosin 1(MYH1) ELISA kit

E07M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Myosin 1(MYH1) ELISA kit

E07M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Myosin 1(MYH1) ELISA kit

E08M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Myosin 1(MYH1) ELISA kit

E08M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Myosin 1(MYH1) ELISA kit

E08M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myosin 1(MYH1) ELISA kit

E01A14713 96T
EUR 700
Description: ELISA

Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

RPU57209-100ug 100ug
EUR 470.4

Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

RPU57209-1mg 1mg
EUR 2184

Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

RPU57209-50ug 50ug
EUR 385

Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

RPD418Hu01 10ug
EUR 176

Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

4-RPD418Hu01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin Heavy Chain 7, Cardiac Muscle, Beta expressed in: E.coli

Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

RPD418Hu02 10ug
EUR 140

Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

RPU42878-100ug 100ug
EUR 542.3

Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

RPU42878-1mg 1mg
EUR 2402.4

Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

RPU42878-50ug 50ug
EUR 435.6

Goat Myosin 1(MYH1) ELISA kit

E01A49621 96T
EUR 700
Description: ELISA

Identification of copy quantity variation of CAPN10 in Thais with kind 2 diabetes by multiplex PCR and denaturing excessive efficiency liquid chromatography (DHPLC).

 Copy quantity variations (CNVs) have been proven to be related to a number of illnesses. They will trigger deviation of genotypes from Hardy-Weinberg Equilibrium (HWE). Genetic case-control affiliation research in Thais revealed that genotype distribution of CAPN10 Indel19 was deviated from HWE after correction of genotyping error. Due to this fact, we goal to establish CNVs inside CAPN10 Indel19 area.

The semi-quantitative denaturating excessive efficiency liquid chromatography (DHPLC) methodology was used to detect CNVs within the area of CAPN10 Indel19 marker in cohort of 305 sufferers with kind 2 diabetes and 250 management topics with out diabetes. CNVs within the area of CAPN10 Indel19 was efficiently detected by DHPLC.

After correction of genotype calling based mostly on the standing of recognized CNVs, CAPN10 Indel19 genotypes had been well-fitted for HWE (p>0.05). Nevertheless, we didn’t discover affiliation between CNV genotypes and threat of kind 2 diabetes in our inhabitants. CNVs in CAPN10 have been recognized in Thais. These CNVs result in deviation from HWE of CAPN10 Indel19 genotypes. After excluding recognized CNVs from the evaluation, CAPN10 Indel19 was related to kind 2 diabetes. The data obtained from our research could be useful for genotyping accuracies of SNPs residing within the CNVs area.

 

Excessive-performance liquid chromatography underneath partially denaturing situations (dHPLC) is a quick and cost-effective methodology for screening molecular defects: 4 novel mutations present in X-linked continual granulomatous illness.

 Implementing exact methods in routine analysis of continual granulomatous illness (CGD), which expedite the screening of molecular defects, could also be important for a fast assumption of affected person prognosis. This research in contrast the efficacy of single-strand conformation polymorphism evaluation (SSCP) and high-performance liquid chromatography underneath partially denaturing situations (dHPLC) for screening mutations in CGD sufferers. We chosen 10 male CGD sufferers with a scientific historical past of extreme recurrent infections and irregular respiratory burst operate. gDNA, mRNA and cDNA samples had been ready by commonplace strategies. CYBB exons had been amplified by PCR and screened by SSCP or dHPLC. Irregular DNA fragments had been sequenced to disclose the character of the mutations.

The SSCP and dHPLC strategies confirmed DNA abnormalities, respectively, in 55% and 100% of the circumstances. Sequencing of the irregular DNA samples confirmed mutations in all circumstances. 4 novel mutations in CYBB had been recognized which had been picked up solely by the dHPLC screening (c.904 insC, c.141+5 g>t, c.553 T>C, and c.665 A>T).

 

This work highlights the relevance of dHPLC, a delicate, quick, dependable and cost-effective methodology for screening mutations in CGD, which in mixture with practical assays assessing the phagocyte respiratory burst will contribute to expedite the definitive analysis of X-linked CGD, direct therapy, genetic counselling and to have a transparent assumption of the prognosis. This technique is very appropriate for creating international locations.

 

dHPLC effectivity for semi-automated cDNA-AFLP analyses and fragment assortment within the apple scab-resistance gene mannequin.

  •  cDNA-AFLP evaluation for transcript profiling has been efficiently utilized to review many plant organic methods, notably plant-microbe interactions. Nevertheless, the separation of cDNA-AFLP fragments by gel electrophoresis is reported to be labor-intensive with solely restricted potential for automation, and the gathering of differential bands for gene identification is much more cumbersome.
  • On this work, we current using dHPLC (denaturing excessive efficiency liquid chromatography) and automatic DNA fragment assortment utilizing the WAVE(®) System to investigate and get better cDNA-AFLP fragments.
  • The tactic is efficiently utilized to the Malus-Venturia inaequalis interplay, making it attainable to gather 66 totally different transcript-derived fragments for apple genes putatively concerned within the protection response activated by the HcrVf2 resistance gene.
  • The outcomes, validated by actual time quantitative RT-PCR, had been according to the plant-pathogen interplay underneath investigation and this additional helps the suitability of dHPLC for cDNA-AFLP transcript profiling.
  • Options and benefits of this new strategy are mentioned, evincing that it’s an nearly absolutely automatable and cost-effective answer for processing massive numbers of samples and amassing differential genes concerned in different organic processes and non-model crops.

Using DHPLC (Denaturing Excessive Efficiency Liquid Chromatography) in II degree screening of the CFTR gene in Prenatal Prognosis.

 OBJECTIVE

The goal of the research is to guage the function of Denaturing Excessive Efficiency Liquid Chromatography (DHPLC) within the second degree screening of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene.

METHODS

A 9-month potential research, between June 2008 and March 2009 at Artemisia Fetal Medical Centre, included 3829 samples of amniotic fluid collected from girls present process mid-trimester amniocentesis.The genetic analysis of CF was based mostly on analysis of the primary mutations of the CFTR gene on fetal DNA extracted from the amniocytes, (first degree screening) utilizing totally different industrial diagnostic methods. A second degree screening utilizing DHPLC, on the amniotic fluid and on a blood pattern from the couple, was provided in case of fetuses heterozygous at first degree screening.

RESULTS

Of 3829 fetuses, 134 had been discovered to be constructive, 129 heterozygous and 5 affected. Of the 129 {couples}, following acceptable genetic counselling, 53 requested a second degree screening. Via using DHPLC, 44 {couples} had been discovered to be destructive, and in 9 {couples}, 9 uncommon mutations had been recognized.

CONCLUSIONS

The primary degree screening will be helpful to proof as much as 75% of the CF mutations. The second degree screening can establish an extra 10% of mutant alleles. DHPLC was discovered to be a dependable and particular methodology for the speedy identification of the uncommon CFTR mutations which weren’t revealed in preliminary first degree screening.

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