Application of DHPLC screening TGFBR-3 gene

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long-qt-syndrome

[Application of DHPLC screening TGFBR-3 gene in Chinese women with idiopathic premature ovarian failure].  OBJECTIVE To judge scientific worth of denaturing excessive efficiency liquid chromatography (DHPLC) utilized in detecting remodeling development issue beta receptor 3 (TGFBR-3) exons 11 and 12 polymorphism in ladies with idiopathic untimely ovarian failure (POF). METHODS From Feb. 2009 to Dec. 2011, 110 […]

COLD-PCR, DHPLC and GeneScanning

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long-qt-syndrome

The use of COLD-PCR, DHPLC and GeneScanning for the highly sensitive detection of c-KIT somatic mutations in canine mast cell tumours.   The conventional polymerase chain reaction (PCR)/sequencing methods may be poorly suited for the detection of somatic mutations in canine mast cell tumour (MCT) samples owing to limited sensitivity. This study was aimed at establishing novel […]

Detection of mutations in gyrB using denaturing

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long-qt-syndrome

Detection of mutations in gyrB using denaturing extreme effectivity liquid chromatography (DHPLC) amongst Salmonella enterica serovar Typhi and Paratyphi A.  Fluoroquinolone resistance is mediated by mutations inside the quinolone-resistance determining space (QRDR) of the topoisomerase genes. Denaturing extreme effectivity liquid chromatography (DHPLC) was evaluated for detection of clinically very important mutations in gyrB amongst Salmonella. […]