Validation of the PCR-dHPLC

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long-qt-syndrome

Validation of the PCR-dHPLC methodology for fast identification of Candida glabrata phylogenetically associated species in several organic matrices. Since two new species phylogenetically associated to Candida glabrata with barely completely different phenotypes and antifungal susceptibility profiles have been described, it appears to be crucial from medical viewpoint, to develop a fast and correct identification system with […]

COLD-PCR, DHPLC and GeneScanning

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long-qt-syndrome

The use of COLD-PCR, DHPLC and GeneScanning for the highly sensitive detection of c-KIT somatic mutations in canine mast cell tumours.   The conventional polymerase chain reaction (PCR)/sequencing methods may be poorly suited for the detection of somatic mutations in canine mast cell tumour (MCT) samples owing to limited sensitivity. This study was aimed at establishing novel […]