Assay Type: Malachite Green Phosphate Detection Kit

Assay duration: 4 hours 40 minutes (after plate preparation)

Sample volume required: 50 µL

Sufficient materials: for fifteen 96-well plates*

Product characteristics

This Malachite Green Phosphate Detection Kit employs a simple, sensitive, reproducible, and non-radioactive method to measure inorganic phosphate in aqueous solutions. The assay is based on the malachite green and molybdate binding reaction and has a wide variety of applications, including the measurement of protein and lipid phosphorylation and phosphatase activity. The protocol presented is for the phosphate assay in a 96-well microplate format. The Malachite Green Phosphate Detection Kit can also be used with 1 mL cuvettes or 384-well plates suitable for high throughput screening.

Kit content

• Malachite Green Reagent A: Ammonium Molybdate in Sulfuric Acid 3M
• Malachite green Reagent B – Malachite green oxalate and polyvinyl alcohol
• phosphate standard

Other necessary reagents

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent