Validation of the PCR-dHPLC methodology for fast identification of Candida glabrata phylogenetically associated species in several organic matrices. Since two new species phylogenetically associated to Candida glabrata with barely completely different phenotypes and antifungal susceptibility profiles have been described, it appears to be crucial from medical viewpoint, to develop a fast and correct identification system with […]
Development of a new DHPLC assay
Growth of a brand new DHPLC assay for genotyping UGT1A (TA)n polymorphism related to Gilbert’s syndrome. BACKGROUND Gilbert’s syndrome is the commonest hereditary dysfunction of bilirubin metabolism. The causative mutation in Caucasians is sort of solely a (TA) dinucleotide insertion within the UGT1A1 promoter. Affected people are homozygous for the variant promoter and have 7 TA repeats […]
Application of DHPLC screening TGFBR-3 gene
[Application of DHPLC screening TGFBR-3 gene in Chinese women with idiopathic premature ovarian failure]. OBJECTIVE To judge scientific worth of denaturing excessive efficiency liquid chromatography (DHPLC) utilized in detecting remodeling development issue beta receptor 3 (TGFBR-3) exons 11 and 12 polymorphism in ladies with idiopathic untimely ovarian failure (POF). METHODS From Feb. 2009 to Dec. 2011, 110 […]
DHPLC/SURVEYOR nuclease: a sensitive, rapid and affordable
DHPLC/SURVEYOR nuclease: a delicate, speedy and inexpensive methodology to investigate BRCA1 and BRCA2 mutations in breast most cancers households. Hereditary breast most cancers accounts for about 10% of all breast cancers and BRCA1 and BRCA2 genes have been recognized as validated susceptibility genes for this pathology. Testing for BRCA gene mutations is normally based mostly […]
COLD-PCR, DHPLC and GeneScanning
The use of COLD-PCR, DHPLC and GeneScanning for the highly sensitive detection of c-KIT somatic mutations in canine mast cell tumours. The conventional polymerase chain reaction (PCR)/sequencing methods may be poorly suited for the detection of somatic mutations in canine mast cell tumour (MCT) samples owing to limited sensitivity. This study was aimed at establishing novel […]
Detection of mutations in gyrB using denaturing
Detection of mutations in gyrB using denaturing extreme effectivity liquid chromatography (DHPLC) amongst Salmonella enterica serovar Typhi and Paratyphi A. Fluoroquinolone resistance is mediated by mutations inside the quinolone-resistance determining space (QRDR) of the topoisomerase genes. Denaturing extreme effectivity liquid chromatography (DHPLC) was evaluated for detection of clinically very important mutations in gyrB amongst Salmonella. […]
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